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Fig. 6 | BMC Cancer

Fig. 6

From: Upregulation of pERK and c-JUN by γ-tocotrienol and not α-tocopherol are essential to the differential effect on apoptosis in prostate cancer cells

Fig. 6

Apoptotic effect of GT3 and AT on prostate cancer cells. a, b, c, and d: SDS total extracts of LNCaP cells treated with (a and c) GT3 or (b and d) AT at doses ranging from 10 to 80 μM were by immunoblot analyzed for the presence of cleaved caspase 9 (a and b) and caspase 3 (c and d). e, f, g, and h: In the same manner as described above, PC3 cells treated with (e and g) GT3 or (f and h) AT, were processed for immunoblot analysis with antibodies against cleaved caspases 9 and 3. The values of each analyzed protein were normalized to β-actin. The values are averages ± standard deviations from at least three independent experiments; *p < 0.05. Autoradiographs of the original blots are available in the Supplementary Section Fig. S9

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