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Fig. 5 | BMC Cancer

Fig. 5

From: Upregulation of pERK and c-JUN by γ-tocotrienol and not α-tocopherol are essential to the differential effect on apoptosis in prostate cancer cells

Fig. 5

Effect on mitochondrial function of GT3 and AT on prostate cancer cells. a and b: LNCaP cells grown on 6-well plates and treated with (a) GT3 or (b) AT for 6 h at doses ranging from 10 to 80 μM were analyzed by immunoblot of SDS total extracts using antibodies against pBAD serine 112. c and d: Cytoplasmic (CF) and mitochondrial fractions (MF) were obtained from LNCaP cells treated with (c) GT3 or (d) AT as described above, and analyzed for the presence of cytochrome C by immunoblot. COX IV was used as a mitochondrial marker. e and f: PC3 cells were grown on 6-well plates and dosed as described above with (e) GT3 or (f) AT. The SDS total extracts were analyzed for the presence of pBAD serine 112 via immunoblot. g and h: PC3 cells dosed with (g) GT3 or (h) AT as described above were processed to obtain cytoplasmic (CF) and mitochondrial fractions (MF) and probed for the presence of cytochrome C and mitochondrial marker COX IV via immunoblot. The values of each analyzed protein were normalized to β-actin. The values are averages ± standard deviations from at least three independent experiments,*p < 0.05. Autoradiographs of the original blots are available in the Supplementary Section Fig. S8.

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