Fig. 4

GT3 and AT have a similar effect on non-tumerigenic prostate cells. a and b: RWPE-1 cells grown on 6-wellplates and treated with (a) GT3 or (b) AT for 6 h at doses ranging from 10 to 80 μM were analyzed by immunoblot using antibodies against the total and phosphorylated forms of ERK. c and d: RWPE-1 cells were treated as described above, and analyzed via immunoblot. SDS total extracts of (c) GT3 or (d) AT treated cells were probed using antibodies against total and activated c-JUN. e and f: The expression levels of total and activated AKT were analyzed via immunoblot in RWPE-1 cells treated with (e) GT3 or (f) AT. The membranes were reprobed for β-actin as al loading control. The values are averages ± standard deviations from at least three independent experiments; *p < 0.05. Autoradiographs of the original blots are available in the Supplementary Section Figure S7