Fig. 3

Involvement of the pERK, pc-JUN and pAKT in the response to treatment with GT3 or AT of PC3 prostate cancer cells. a, c, and e: PC3 cells were grown on 6-well plates and treated with GT3 in the same manner as described above. Immunoblots were obtained from SDS total extracts by using antibodies against the total and phosphorylated forms of ERK (a), c-JUN (c), and AKT (e). b, d, and f: Immunoblots of SDS total extracts obtained from PC3 cells treated with AT as described above, were obtained using antibodies against the phosphorylated and total forms of ERK (b) c-JUN (d), and AKT (f). Cells that were treated with neither GT3 nor AT, only with dissolution vehicle, were run as controls for each experiment. The membranes were reprobed for β-actin as a loading control. The results presented are representative of three separate experiments. The values are averages ± standard deviations from three independent experiments;*p < 0.05. Autoradiographs of the original blots are available in the Supplementary Section Figure S6.