Fig. 2

Involvement of the pERK, pc-JUN and pAKT in the response to treatment with GT3 or AT of LNCaP prostate cancer cells. a, c, and e: LNCaP cells were grown on 6-wellplates and treated with GT3 for 6 h at doses ranging from 10 to 80 μM. Immmunoblots from SDS total extracts were obtained using antibodies against total and phosphorylated forms of ERK (a) (c) c-JUN, and (e) AKT. b, d, and F: Similarly, LNCaP cells treated with AT for 6 h at the same dose range as described above. Immunoblots from total SDS extracts using antibodies against the total and phosphorylated forms of ERK (b), c-JUN (d) and AKT (f) are shown above. The membranes were reprobed for β-actin as a loading control. The results presented are representative of three separate experiments. The values are averages ± standard deviations from three independent experiments;*p < 0.05, **p < 0.01. Autoradiographs of the original blots are available in the Supplementary Section Figure S5.