Fig. 4

Translational validation of AHCY gain in the resistant clone, and metabolomics analysis. a IHC results from BLUE-1, BLUE-1 K12 and BLUE-1R12 cell lines. Sections from FFPE cell line blocks were stained with anti-AHCY antibody. b Validation of AHCY overexpression at the protein level using Western blot. Whole cell protein lysates from the cell lines were analyzed using Western blot. Histone 3 was used as a loading control for the blot. The full-length blot is presented in Additional file 7: Fig. S6. FUSION-CAP Software was used for Western blot image analysis. c Quantification of S-adenosyl methionine, adenine and adenosine in BLUE-1, BLUE-1 K12 and BLUE-1R12. Values are shown as mean plus SD from six replicate measurements