Fig. 3

Immunofluorescence and hematoxylin and eosin staining. a Representative images of vimentin, CK19, CA 19–9, e-cadherin and p53 expression of primary cell cultures with organoid formation and corresponding tumors, indicated by IF staining. Scale bars, 20 μm. b IF staining of vimentin, CK19 and CA 19–9 expression of two dimensional primary cell cultures (representative images). Scale bars, 20 μm. c HE staining of primary tumors (representative image). Scale bars, 100 μm