Fig. 3

Functional role of FAT4 on cell proliferation, colony formation, and invasive ability. a. Alamar blue assay displaying enhanced cell proliferation in FAT4 knocked MCAS and OVSAHO cells (p = 0.0001 and p = 0.0054 respectively) as compared to control. b. Colony formation assay in control and FAT4 siRNA treated MCAS, and OVSAHO cells. The assay shows a higher number of colonies in FAT4 siRNA treated cells. The cells were stained with crystal violet. c. Soft agar colony formation assay in control and FAT4 siRNA treated MCAS and OVSAHO cells. Anchorage-independent growth was significantly higher in cells treated with siFAT4 MCAS cells (p = 0.0055) and OVSAHO cells (p = 0.0302). d. Silencing FAT4 by siRNA promoted cell invasion in vitro in MCAS and OVSAHO cells (p = 0.0001 in MCAS and p = 0.003 in OVSAHO). MCAS and OVSAHO cells treated with scrambled siRNA were used as control. Data represent the mean and the standard deviation from at least three independent experiments performed in triplicates