Fig. 4

Tripolin A reversed the promotion effect of over-expressed Prdx1 on p-Aurora A and HDAC6. a Western-blot was used to detect changes of Aurora A expression in EC9706 cells treated with different concentrations (0, 1.5, 4.5, 7.5, and 10.5 μmol/L) of Tripolin A. b Relative gray level for p-Aurora A/Aurora A in cells after treatment with different concentrations of Tripolin A. c Changes of p-Aurora A/Aurora A expression in EC9706 cells treated with 4.5 μmol/L Tripolin A for different lengths of time (0, 2, 4, 8, and 12 h). d Relative gray level of p-Aurora A/Aurora A in cells after treatment with 4.5 μmol/L Tripolin A for different lengths of time. e The expression of NEDD9, p-Aurora A, Aurora A, and HDAC6 after EC9706 cells treated with 4.5 μmol/L Tripolin A for 2 h. f Analysis of western blot signals using Image J. GAPDH was used as a loading control. g The expression of NEDD9, p-Aurora A, Aurora A, and HDAC6 in different treatment groups. The OE-Prdx1 group was EC9706 cells transfected with the OE-Prdx1 lentivirus, and then treated with 4.5 μmol/L Tripolin A for 2 h. h Relative expression of NEDD9, p-Aurora A and HDAC6 in cells of different groups. GAPDH was used as a loading control. Full-length blots/gels are presented in Supplementary Fig. X. *P < 0.05, **P < 0.01, ***P < 0.001