Fig. 1

Impact of RA190 upon HepG2 cell viability, colony formation, polyubiquitinated protein, and apoptotic markers. a HepG2 cells were plated at 96/well of a microtiter plate in the presence of titrations of RA190 or sorafinib, and after 72 h the cell viability was determined by MTT assay. b Treatment of HepG2 cells with RA190 reduced the number tumor colonies in a clonogenicity assay. c HepG2 cells after 12 h treatment with 0, 1, or 2 μM RA190 were harvested and subjected to Western blot analysis with antibody to Ubiquitin, PARP and Actin (full-length blots/gels are presented in Fig. S4). d HepG2 cells were treated with 2 μM RA190 and harvested at the indicated times. Cell lysates were subjected to Western blot analysis with antibody to Ubiquitin, p21 and Actin (full-length blots/gels are presented in Fig. S5)