Fig. 1

HIF1a overexpression in the androgen dependent LNCaP cell line increased proliferation and resistance to androgen deprivation therapy. a, stable HIF1a expression increased cell proliferation compared to the LNCaP/Empty control cells when cells were treated with the ethanol vehicle control or synthetic androgen R1881 (two way multiple comparison ANOVA; *p < 0.05, **p < 0.01). b, stable HIF1a expression led to resistance to bicalutamide treatment (two-tail t-test, * p = 0.058). Data points represent the mean of three intra-assay and two biological repeats ± SEM. Differences in growth rates are due to cells grown in media containing charcoal stripped serum (androgen depleted media) for 96 h prior to treatment (a) or in standard RPMI median with FBS that contains androgen and growth stimulants (b)