Fig. 4
From: A novel pancreatic tumour and stellate cell 3D co-culture spheroid model
Histological and immunohistochemical characterization of HPAFII and hPSC mono- and heterospheroids cultured for 5 days. Haematoxylin and eosin-stained central sections of mono- and co-cultured HPAFII/hPSC spheroids (a). Immunohistochemical double-staining for the epithelial marker CK19 (red) and the proliferation marker MKI67 (brown) (b). CK19/MKI67 double staining was also used to determine the percentage of HPAFII cells (CK19+)/hPCS cells (CK19−) (c) and the percentage of proliferating (MKI67+) HPAFII and hPSC cells (d) in mono- and heterospheroids, corresponding to mono-culture (MC) and co-culture (CC), respectively, over a period of 7 days. Black scale bars in a) and b) correspond to 200 μm