Fig. 2
Detection sensitivity for BRAFV600E mutation in mRNA. The sensitivity of a novel mRNA based mutation assay for BRAFV600E was determined using 107 copies of in vitro transcribed mRNA containing the BRAFV600E mutation and the same amount of corresponding wildtype mRNA as templates: a Amplification signal from mutant BRAFV600E mRNA (red line), wildtype BRAF mRNA (blue line) and no-template control-NTC (green line); b) Corresponding melting peaks of the amplification products