Fig. 1
Overview of the BRAFV600E mRNA mutation detection assay. Mutant BRAFV600E mRNA was detected in a two-step qPCR reaction as follows: I) A mutation-specific reverse transcription, utilizing a warmstart reverse transcriptase that is activated at relatively high temperature (40o-50 °C), in combination with an extendable wildtype-blocking probe and a 5′-tailed BRAFV600E mutation-specific primer; II) selective qPCR amplification of cDNA derived from mutant BRAFV600E mRNA
