Table 1 Monoclonal antibodies used in IHC staining
From: MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers
Antibody | Clone | Concentration | Target cells | Company |
|---|---|---|---|---|
ER | SP1 | 1:400 | Epithelial cells | Thermo Scientific, Waltham, USA |
CD4 | 4B12 | 1:100 | CD4+ T-cells | Novocastra, Newcastle Upon Tyne, UK |
CD8 | C8/144B | 1:50 | CD8+ T-cells | DAKO, Agilent Technologies, Santa Clara, CA, USA |
CD20 | L26 | 1:1000 | B-cells, neoplasms of B-cells | DAKO |
CD68 | PG-M1 | 1:400 | B-cells, macrophages, histiocytes, dendritic cells, NK cells | DAKO |
CD138 | B-A38 | 1:50 | Plasma cells and some epithelial cells | AbD Serotec (BioRad), Kidlington, UK |
PAX5 | 24 | 1:100 | B-cells | BD Biosciences, San Diego, USA |
Actin | 1A4 | 1:300 | Fibroblasts | DAKO |