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Fig. 4 | BMC Cancer

Fig. 4

From: Roles of DANCR/microRNA-518a-3p/MDMA ceRNA network in the growth and malignant behaviors of colon cancer cells

Fig. 4

Silencing of miR-518a-3p partially reverses the inhibition on CC cell behaviors induced by DANCR down-regulation. miR-518a-3p inhibitor and its mock plasmid were transfected into si-DANCR-treated HT29 and SW116 cells. a, miR-518a-3p expression in cells measured using RT-qPCR; b, proliferation of HT29 and SW116 cells measured via EdU assay; c, viabilities of HT29 and SW116 cells detected using MTT assay; d-e, apoptosis of HT29 and SW116 cells evaluated using Hoechst 33258 staining (D) and flow cytometry; f, protein levels of EMT markers Snail, Vimentin and E-cadherin in cells determined by western blot analysis (See original images in Supplementary Figure S3); g, invasion and migration abilities of HT29 and SW116 cells assessed via Transwell assays. Data are expressed as mean ± SD; in panels A, B, D, E and G, data were analyzed using one-way ANOVA, while data in panels C and F were analyzed via two-way ANOVA, and Tukey’s multiple comparison test was applied for the post hoc test; *, p < 0.05

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