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Fig. 3 | BMC Cancer

Fig. 3

From: Roles of DANCR/microRNA-518a-3p/MDMA ceRNA network in the growth and malignant behaviors of colon cancer cells

Fig. 3

DANCR regulates MDM2 expression via interacting with miR-518a-3p. a, subcellular localization of DANCR predicted via the Lncatlas database; b, nuclear and cytoplasmic DANCR expression in HT29 and SW116 cells determined by RT-qPCR; c, FISH experiments with probes targeting DANCR were performed to validate the subcellular localization of DANCR in HT29 and SW116 cells, the cytoplasm was stained with probes targeting DANCR (green stain), and the nuclei were stained with DAPI (blue stain); d, binding relationship between DANCR and miR-518a-3p were predicted on StarBase, and luciferase reporter plasmids containing DANCR-WT or DANCR-MUT were co-transfected into H293T cells with miR-NC plasmid as NC; e, miR-518 expression in HT29 and SW116 cells following DANCR inhibition detected using RT-qPCR; f, binding relationship between miR-518a-3p and MDM2 were predicted on StarBase luciferase reporter plasmids containing MDM2-WT or MDM2-MUT were co-transfected into H293T cells with miR-NC plasmid as NC; g, enrichment of miR-518a-3p on the MDM2 mRNA detected by RNA pull down-qPCR assay, relative to antisense-oligos; h-i, relative mRNA expression (H) and protein level (I) of MDM2 following DANCR inhibition determined by qRT-PCR and western blot analysis (See original images in Supplementary Figure S2), respectively; j-k, expression of miR-518a-3p and MDM2 in HT29 and SW116 cells and in FHC cells determined by RT-qPCR. Data are expressed as mean ± SD; in panels D, E, G, H, J and K, data were analyzed using one-way ANOVA, while data in panels B, I and F were analyzed via two-way ANOVA, and Tukey’s multiple comparison test was applied for the post hoc test; *, p < 0.05, **, p < 0.01

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