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Fig. 2 | BMC Cancer

Fig. 2

From: Impact of nuclear YAP1 expression in residual cancer after neoadjuvant chemohormonal therapy with docetaxel for high-risk localized prostate cancer

Fig. 2

The effects of YAP1 knockdown on cell proliferation, induction of apoptosis and modulation of the cell cycle phase. a 22Rv1-DR cells were treated with 25 nM siYAP1 or siControl for 24–48 h. The day after treatment, total cellular RNA was extracted, and YAP1 mRNA expression was analyzed by quantitative RT-PCR. The YAP1 mRNA levels of the cells treated with siRNAs were normalized to the mRNA levels of the untreated 22RV-1-DR cells. **p < 0.05. b 22Rv1-DR cells were treated with 25 nM of siYAP1 or siControl for ≤6 days. Cell viability was determined by MTT-based cell proliferation assay. The cell number after siRNA induction was compared with that of the untreated 22Rv1-DR cells. *p < 0.05. c, d 22Rv1-DR cells were treated with 25 nM siRNAs for 24 h (c) and 48 h (d), and then harvested for flow cytometric analyses. The fractions of each cell cycle phase were compared between the cells treated with siYAP1 and those treated with control siRNA

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