Fig. 1

Establishment of docetaxel-resistant 22Rv1 subclones and Hippo pathway protein expression. a Cell proliferation of 22Rv1 and 22Rv1-DR cells. A total of 1.0 × 104 cells were seeded into each well of a 24-well plate and incubated for 72 h containing 10% FBS. Cell proliferation was determined using a non-radioactive MTT-based cell proliferation assay kit. b PARP protein expression in the 22Rv1 cell lines with different docetaxel concentrations and timing were measured by western blotting. β-actin was used as a loading control. c MDR-1 protein expression in the 22Rv1 and 22Rv1-DR cell lines was measured by western blotting. 22Rv1-DR cells were cultured for 4 months in medium with docetaxel. β-actin was used as a loading control. d Whole and nuclear expression of Hippo pathway proteins.β-actin was used as a loading control for whole cell lysates, whereas Lamin A/C was used as a loading control for nuclear lysates. Blotting signals were captured using CS Analyzer 3.0 software (ATTO). The blots in the figure were cropped, but the polyacrylamide gels were run under the same experimental conditions. The original gel images were presented in Supplementary Figure 3, 4 and 5