Fig. 5

SIRT3 induction after PD0332991 treatment. a HepG2, Hep3B, SK-Hep1, and Huh7 cells were incubated with DMSO, 1 μM PD0332991, or 10 μM PD0332991. After 48 h, SIRT3 and actin levels were evaluated using western blotting. The images shown here are cropped and the full-length blots/gels are presented in Additional file 2: Fig. S5. b-d HepG2 cells (b), Huh7 cells (c), SK-Hep1 cells (d) were transfected with scrambled siRNA oligos or siRNA oligos against CDK4/6. After 48 h, western blotting was performed to detect indicated proteins. The images shown here are cropped and the full-length original blots are shown in Additional file 2: Fig. S6, S7 and S8. e Fold change of the indicated genes obtained by gene expression profiling of HepG2 cells after transfection with scrambled siRNA oligos or siRNA oligos targeting CDK4/6. All data are uploaded into the GEO database under the accession number GSE145389