Fig. 4

Negative correlation between SIRT3 and retinoblastoma protein (Rb). a Formalin-fixed, paraffin-embedded (FFPE) liver tissues from HCC xenograft model were used. Immunohistochemistry was performed using antidies against phosphor-Rb (pRb) and counterstained with hematoxylin. Scale bars: 50 μm. b RNA was extracted from frozen HCC samples obtained after transsphenoidal surgery. RT-PCR was performed using probe sets specific for Rb. The expression of the target genes was normalized to that of B2M (housekeeping gene) using the 2^−ΔΔCt method. The boundary of the box closest to zero indicates the 25th percentile, the line within the box marks the median, and the boundary of the box farthest from zero indicates the 75th percentile. *P < 0.05. c FFPE liver tissues from human patients with HCC were used. Immunohistochemistry was performed using antibodies against SIRT3 (left) and pRb (right) and counterstained with hematoxylin. Scale bars: 50 μm