Fig. 2

Expression of SIRT3 in hepatocellular carcinoma (HCC) cell lines and HCC xenograft models. aSIRT3 expression in four different HCC cell lines was measured using quantitative RT-PCR. The expression level of target genes was normalized to that of the housekeeping gene B2M using the 2^−ΔΔCt method. Data are shown as the mean of three independent experiments ± SD. b Western blotting in different HCC cell lines using antibodies against SIRT3 and actin. The images shown here are cropped and the full-length blots/gels are presented in Additional file 2: Fig. S1. c Formalin-fixed, paraffin-embedded liver tissues from HCC xenograft model were used. Immunohistochemistry was performed using antibodies against SIRT3, GLUT1, and Ki67 and counterstained with hematoxylin. Scale bars: 20 μm. d Huh7 cells were transfected with MOCK vector and pcDNA-SIRT3. After 48 h of incubation, protein was extracted and the expression of SIRT3, Ki67, and actin was determined using western blotting. The images shown here are cropped and the full-length blots/gels are presented in Additional file 2: Fig. S2. e Glucose uptake was measured using Glucose-Glo Assay. Data are shown as the mean of three independent experiments ± SD. Statistical analyses were performed using GraphPad PrismComparisons between groups were made using the Mann-Whitney test. *P < 0.01