Fig. 1

SIRT3 expression in patients with hepatocellular carcinoma (HCC) and with different 18F-FDG uptake. a. RNA was extracted from frozen HCC samples obtained after transsphenoidal surgery. RT-PCR was performed using probe sets specific for SIRT3. The expression of the target genes was normalized to that of B2M (housekeeping gene) using the 2^−ΔΔCt method. The boundary of the box closest to zero indicates the 25th percentile, the line within the box marks the median, and the boundary of the box farthest from zero indicates the 75th percentile. b. Formalin-fixed, paraffin-embedded human HCC samples were used and immunofluorescence was performed using the indicated antibodies and counterstained with DAPI. Scale bars: 50 μm. Statistical analyses were performed using GraphPad Prism. Results are expressed as mean ± SD. Comparisons between groups were made using the Mann-Whitney test. *P < 0.05