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Fig. 4 | BMC Cancer

Fig. 4

From: Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours

Fig. 4

Effects of manipulating Nodal and Activin signalling in GCNIS cells cultured ex vivo for 4 days. a) Immunohistochemical staining with BrdU (proliferation marker), cPARP (apoptosis marker) and OCT4 (pluripotency marker) in adult testis tissue containing GCNIS cells treated with SB431542 (20 μM), recombinant Nodal (50 ng/ml) or vehicle for 4 days. Sections were counterstained with Mayer’s haematoxylin. Scale bar corresponds to 100 μm. Quantification of the number of b) BrdU+ cells per mm2, c) cPARP+ cells per mm2 and d) OCT4+ cells per mm2. Number of positive cells is normalised to vehicle controls (set to 1), and only apoptotic GCNIS cells were quantified. Tissue from three patients was evaluated (n = 3). Top panel of b-d) shows results from the individual patient samples, while bottom panel represents mean ± SEM

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BMC Cancer

ISSN: 1471-2407

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