Fig. 2

Effects of manipulating Nodal and Activin signalling separately in NTera2 cells. Expression of pluripotency factors (OCT4, NANOG) and Nodal signalling factors (NODAL, CRIPTO, LEFTY1) in NTera2 cells following a) 48 h and b) 7 days of treatment with recombinant Nodal (50 ng/ml) and Lefty (100 ng/ml) and c) 48 h and d) 7 days of treatment with Activin A (50 ng/ml) and Follistatin (100 ng/ml). RPS20 was used as reference gene. Expression level is set to 1 in the vehicle controls. Experiments were conducted in triplicates in three independent experiments (n = 3) and measured as technical triplicates. Values represent mean ± SEM. Significant difference compared to expression in vehicle control-treated cells, * P < 0.05, ** P < 0.01 and *** P < 0.001. Note logarithmic scale