Fig. 1

Effects of simultaneously inhibiting Nodal and Activin signalling in the NTera2 cell line. NTera2 cells were treated with 20 μM, 10 μM and 5 μM SB431542 for a) 24 h and b) 48 h followed by assessment of cell proliferation. Experiments were set up with 16 replicates per treatment and repeated in three independent experiments (n = 3). Cell proliferation is set to 1 in the vehicle controls. Expression of pluripotency factors (OCT4, NANOG) and Nodal pathway genes (NODAL, CRIPTO, LEFTY1) in NTera2 cells following c) 48 h and d) 7 days of treatment with SB431542 (40 μM, 20 μM and 4 μM). RPS20 was used as reference gene. Expression level is set to 1 in the vehicle controls. Experiments were conducted in triplicates in three independent experiments (n = 3) and measured as technical triplicates. Values represent mean ± SEM. Significant difference compared to expression in vehicle control-treated cells, * P < 0.05, ** P < 0.01 and *** P < 0.001. Note logarithmic scale