Fig. 7
From: Drug resistance occurred in a newly characterized preclinical model of lung cancer brain metastasis
In vitro characterization of PC-9 Parental and PC-9-Br. a Cytotoxic effects of gefitinib on PC-9-Br and PC-9 parental at 48 h and 72 h. Data are expressed as the percentage by comparing vehicle control determined by the MTT assay. Values are represented as mean ± SD, n = 6. b Gene analyses of PC-9-Br showing no T790M (c.2369C > T) was found in EGFR exon 20 of PC-9-Br. c Western blot analyses of EGFR, p-EGFR (Y1068), and EMT biomarkers (E-cadherin and Vimentin) in PC-9 parental and PC-9-Br. d qRT-PCR analyses of PC-9-Br compared to PC-9 parental. Data are mean ± SD. “*” indicates a significant difference between PC-9-Br and PC-9 parental analyzed by a Student’s t test (p < 0.05). Full western blot images are presented in supplementary figure1. The Bio-Rad ChemiDoc™ imaging system was used for western blot image acquisition