Fig. 5

Passive permeability changes of PC-9-Br treated with cisplatin+etoposide. a A representative cresyl violet brain slice (approximately corresponding to Fig. 2 C1) of cisplatin+etoposide-treated PC-9-Br tumors, with b corresponding Tomato Red tumor fluorescence. c The same slice with Oregon Green, d ICG, and e¹⁴C-AIB autoradiographic data to quantify P-gp, vascularity, and permeability increases, respectively. f The median and interquartile ranges for fold-increases of dyes in 117 tumors over control regions. For cisplatin-etoposide-treated brains, tumors were significantly more permeable ¹⁴C-AIB (red) and OG (green) and ICG (blue) than control regions (p < 0.05). g The fold increases of OG (green), ICG (blue), or ¹⁴C-AIB (red) were not correlated with metastases size (r² < 0.02). For all depicted brain slices, tumor regions are outlined while control areas are squares. Scale bar = 1 mm