Fig. 2

Analysis of the subcellular localization of E618 and DLG1 in co-expression experiments. a Subcellular distribution of E618 and DLG1 in epithelial cells. The encoding vectors for seyfp2-E618 or mTurq2-DLG1 were separately transfected into HEK293 cells and the localization of each fusion protein was analysed by confocal microscopy (yellow arrows). b DLG1 is barely expressed in the presence of high levels of E618. A 5:1 ratio of pseyfp2-E618:pmTurq2-DLG1 plasmids were used for transfection. Yellow arrows indicate the fusion protein localization. c Co-localization analysis of E618 with DLG1. A ratio of 1:2 of pseyfp2-E618:pmTurq2-DLG1 plasmids were used for transfection. White arrows indicate E618 redistribution. Light blue arrows indicate cellular regions where fusion proteins colocalize (see Merge image). PCC was calculated to evaluate seyfp2-E618/mTurq2-DLG1 colocalization, analysing a total of 90 micrographs from four independent experiments (right). d Analysis of E618 ΔPBM and mTurq2-DLG1 co-expression in epithelial cells. For the microscopy examination the same plasmid ratio as in (c) was used to co-transfect HEK293 cells. The yellow arrow shows the expression of seyfp2-E618ΔPBM in the nucleus. All scale bars represent 10 μm. The results are representative of at least 5 independent experiments