Fig. 1

DLG1 and E618 protein levels are dependent on their relative abundance. a DLG1 levels decrease when the E618 oncoprotein is expressed at high levels. Different pseyfp2-E618/pmTurq2-DLG1 plasmid DNA ratios were transfected into HEK293 epithelial cells (5:1 and 1:1 μg). pcDNA3 was used as empty vector control. Middle and Right panels, densitometry analysis of western blots for DLG1 expression for equal γ tubulin levels in the different E6:DLG1 ratio conditions (mean ± SD, n = 3). Asterisks denote significant difference tested by one-tailed t-test (*p < 0.05). b High levels of DLG1 promote an increment in E618 levels. A 1:2 μg ratio of pseyfp2-E618/pmTurq2-DLG1 plasmid was used. pcDNA3 was used as empty vector control. Right panel, densitometry analysis of western blots for E618 expression for equal γ tubulin levels (mean ± SD, n = 3). Asterisks denote significant difference among conditions tested by one-tailed t-test (*p < 0.05). The results shown are representative of three independent experiments. In all cases, cells were harvested 24 h post-transfection and the expression of fusion proteins was assessed by western blot using an anti-GFP antibody, which recognizes all fluorescent tagging proteins. β-Gal acted as a control for transfection efficiency and γ tubulin levels as loading control. Full-length blots are presented in the Supplementary figure S3