Fig. 2

SLC6A1 overexpression promotes proliferation, migration and invasion of prostate cancer cell lines PC3 and LNCaP. a Both qPCR and Western blot analyses confirmed that the cell lines were successfully established. We choose the PC3-sh-SLC6A1-c and LNCaP-sh-SLC6A1-c to establish cell lines PC3 and LNCaP with the specific shRNA. Full-length blots/gels are presented in Supplementary Figure 5, 6, 7, 8. b Cell proliferation of PC3 and LNCaP cells transfected with SLC6A1 expression vector or the specific shRNA examined by CCK-8 assay.c Cell cycle distribution of PC3 and LNCaP cells transfected with SLC6A1 expression vector or the specific shRNA examined by flow cytometry analysis. The chart showed the number of cell cycle distribution in different stages of PC3 and LNCaP cell lines. Histogram showing the percentages of cells in each phase of the cell cycle which was analyzed by ModFit LT (Verity Software). d Invasion activity of PC3 and LNCaP cells transfected with SLC6A1 expression vector or the specific shRNA examined by Transwell assays. e Migration activity of PC3 and LNCaP cells transfected with SLC6A1 expression vector or the specific shRNA examined by Wound-healing assays