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Fig. 2 | BMC Cancer

Fig. 2

From: LncRNA SNHG7 promotes the proliferation of nasopharyngeal carcinoma by miR-514a-5p/ELAVL1 axis

Fig. 2

SNHG7 sponges miR-514a-5p in NPC. a Subcellular fractionation assay detected the distribution of SNHG7 in C666–1 and HNE1 cells. b starBase website screened out two miRNAs that possess the highest binding ability with SNHG7. c RNA pull down assay analyzed the interaction between SNHG7 and miR-668-3p (or miR-514a-5p). d The expression of miR-514a-5p in NPC cells and nasopharyngeal epithelial cell line was quantified by RT-qPCR. e The influences of SNHG7 silencing on miR-514a-5p expression were evaluated via RT-qPCR. f RIP assay proved that SNHG7 and miR-514a-5p coexisted in RISC. g The potential binding sites between SNHG7 and miR-514a-5p were displayed. h The overexpression efficiency of miR-514a-5p was quantified by RT-qPCR. i Luciferase activity assay demonstrated that SNHG7 could bind with miR-514a-5p. GAPDH or U6 was used as an internal control. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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