Skip to main content
Fig. 4 | BMC Cancer

Fig. 4

From: lncRNA CRNDE promotes the proliferation and metastasis by acting as sponge miR-539-5p to regulate POU2F1 expression in HCC

Fig. 4

The promoting effects of lncRNA CRNDE can be reversed by miR-539-5p and POU2F1. a qRT-PCR analysis of miR-539-5p expression in three HCC cells and one cultured human liver epithelial cells. b qRT-PCR analysis of POU2F1 expression in three HCC cells and one cultured human liver epithelial cells. Western Blot analysis of POU2F1 expression in three HCC cells and one cultured human liver epithelial cells. c We divided the cells into groups: pcDNA3/lncRNA CRNDE, miR-539-5p mimics, pcDNA3, transfected for 48 h. MTT assays were performed to detect the proliferation ability of QGY-7703 and HepG2 cells. d We divided the cells into groups: miR-539-5p mimics, pcDNA3/POU2F1, pcDNA3, transfected for 48 h. MTT assays were performed to detect the proliferation ability of QGY-7703 and HepG2 cells. e, f We divided the cells into three groups: pcDNA3/lncRNA CRNDE, miR-539-5p mimics, pcDNA3, transfected for 48 h. Transwell migration and invasion assays were performed to detect the migration and invasion ability of QGY-7703 and HepG2 cells. *p < 0.05, **p < 0.01. All bars indicate the means±SD. All experiments were performed independently at least three times. g, h We divided the cells into groups: miR-539-5p mimics, pcDNA3/POU2F1, pcDNA3, transfected for 48 h. Transwell migration and invasion assays were performed to detect the migration and invasion ability of QGY-7703 and HepG2 cells. *p < 0.05, **p < 0.01. All bars indicate the means±SD. All experiments were performed independently at least three times

Back to article page