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Fig. 3 | BMC Cancer

Fig. 3

From: lncRNA CRNDE promotes the proliferation and metastasis by acting as sponge miR-539-5p to regulate POU2F1 expression in HCC

Fig. 3

lncRNA CRNDE acted as a ceRNA by sponging miR-539-5p and regulated POU2F1 expression indirectly. We divided the cells into four groups: mimics NC, miR-539-5p mimics, inhibitor NC, and miR-539-5p inhibitor, transfected for 48 h, and then performed subsequent experiments. a The putative target sites between lncRNA CRNDE and miR-539-5p were shown. b Dual-luciferase analysis was proformed when HCC cells were co-transfected with lncRNA CRNDE-wt and miR-539-5p mimic or lncRNA CRNDE-mut and miR-539-5p mimic. The activity of luciferase was detected. c miR-539-5p expression level was detected by qRT-PCR when lncRNA CRNDE was overexpressed or knocked down. d RNA pulldown was used to presented the binding between lncRNA CRNDE and miR-539-5p as fold enrichment. e Prediction program was used to find that there is a putative target of miR-539-5p in the POU2F1 sequences. f Dual-luciferase analysis showed that effects of miR-539-5p on the luciferase activity of constructs of the type binding site. qRT-PCR (g) and Western blot (j) were used to detect the POU2F1 level when the miR-539-5p was overexpressed or knocked down. miR-539-5p. k Immunofluorescence was used to detect the expression of POU2F1 in HepG2 cells. qRT-PCR (l) and Western blot (m) were used to detect the level of miR-539-5p and POU2F1 in nude tissues. o and POU2F1 (p) relative expression levels were determined in 18 paired HCC clinical tissues and their corresponding normal samples.*p < 0.05, **p < 0.01. All bars indicate the means±SD. All experiments were performed independently at least three times

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