Fig. 5

CHD4 down-regulation suppressed the RhoA/ROCK signaling pathway by interacting with PHF5A. a CHD4 down-regulation decreased RhoA, ROCK, p-myosin and p-ERK, but increased E-cadherin expression in both H1299 and A549 cell lines. (left and up), and the results of which were further quantified (right and down). β-actin served as a loading control. Full-length blots were presented in Supplementary Fig. 4. b The co-immunoprecipitation (IP) analysis of the CHD4 and PHF5A association in human A549 cells. Full-length blots were presented in Supplementary Fig. 5. c CHD4 suppression decreased the expression of PHF5A, while down-regulation of PHF5A could reduce RhoA, ROCK, rather than CHD4 expression in A549 cells (left), and the results of which was further quantified (right). β-actin served as a loading control. Full-length blots were presented in Supplementary Fig. 6 The results were shown as the mean ± S.D.; P < 0.05 was considered significant