Fig. 2

The effects of CHD4 down-regulation on NSCLC cell proliferation and migration. a Verification of siRNA-mediated knockdown of the CHD4 gene in A549 and H1299 cells by western blotting. Full-length blots were presented in Supplementary Fig. 2A. b The effects of siRNA-mediated knockdown of CHD4 on the proliferation of A459 and H1299 cells determined by MTT assay. c Representative results of the cell cycle analyses by FACS. CHD4 arrested the cell cycle in G1/S phase in A459 and H1299 cells (left). Quantification of the results of the cell cycle analysis was shown on the right. d Representative results of trans-well migration assays at 24 h after CHD4 knockdown in the A549 and H1299 cells. Statistical analysis of the differences between the groups was performed with an unpaired Student’s t-test. The results were shown as mean ± s.e.m., *, P < 0.05; **, P < 0.01; ***, P < 0.001