Fig. 6

Analysis of cytokines in VP-treated cells: Cytokine levels in control and VP-treated samples were determined using human cytokine antibody array as per manufacturer instructions. Using this array, we performed the proteomic analysis of cytokines and screened the expression of 80 cytokines in OVCA cell lines. The membranes were incubated with cell lysates, then processed and assayed using chemiluminescence technique. Spots were identified and based on the signal intensities; relative levels of cytokines were established