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Fig. 2 | BMC Cancer

Fig. 2

From: Synergistic enhancement of efficacy of platinum drugs with verteporfin in ovarian cancer cells

Fig. 2

Effect of VP sensitization on cytotoxicity of A2780Cis cells: a. Bar graph showing cytotoxicity after chemosensitization. Cells were counted and seeded in 96-well plates (5000cells/well). Treatment conditions: Drugs alone: Cells were grown in fresh medium for 24 h and then drugs were added. VP Sensitization: Cells were treated with VP (Fa0.1) for 6 h. After 6 h, cells were washed with fresh medium 3X, lifted, counted and seeded in 96-well plates (5000cells/well). Cells were grown in fresh media for 1d/2d/3d/7d and then drugs were added at Fa0.1. Drug treatments are given for 24 h. Cytotoxicity values were based on cell viability assays (CellTiter-Blue®). Bars represent Mean ± SEM. n = 6. * Significant at p < 0.05 (1-way ANOVA, Control vs drug dose). VP = Verteporfin; CDDP = Cisplatin; CP = Carboplatin; Taxol = Paclitaxel. b. Actual IC50 values of A2780Cis cells. c. Concentrations of drugs used for the assay. d. Western blots showing the effect of drugs on YAP and ABCG2 in A2780Cis cells. Equal amounts of proteins (40 μg) from untreated and treated A2780Cis cell lysates were loaded on 10% gels and transferred onto nitrocellulose membranes, which were then probed with respective antibodies. GAPDH was used a positive loading control. n = 3

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