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Table 1 Immunohistochemistry conditions

From: Stem/progenitor cell marker expression in clear cell renal cell carcinoma: a potential relationship with the immune microenvironment to be explored

Target

Antibody (Catalogue)

Pre-treatment

Dilution

Incubation

CD44

AbCam (ab157107)

no pretreatment

1/500

1 h

CD90

AbCam (133350)

Citrate pH 6.0

1/300

1 h

CD146 (MCAM)

AbCam (75769)

Tris-EDTA pH 9.0

1/800

1 h

ST3GAL2

Novus (NBPI87044)

Citrate pH 6.0

1/100

Overnight