Fig. 1
From: Therapeutic implications of PD-L1 expression in bladder cancer with squamous differentiation
PD-L1 protein expression in squamous differentiated bladder cancer (SD-BLCA). a Immunohistochemical PD-L1 staining is shown for representative tissue cores illustrating both immune cells (IC) and tumour cells (TC) by applying four different antibodies: DAKO 28–8, DAKO 22C3, Ventana SP263 and Ventana SP142. Squamous tumour components are histologically shown by H&E staining. CD68 staining highlights macrophages. Black scale bar: 100 μM. Please note: Due to tissue loss during the first immunohistochemical staining with the 22C3 antibody deeper tissue sections of the patient’s FFPE material were used which show slight differences in histology. b-c Scatter plot graphs show overall distribution of PD-L1 positive areas of IC and TC for mixed (UC/SCC) and pure squamous cancers (SCC). d-e Spearman correlation analysis demonstrating inter-assay heterogeneity for IC (d) and TC (e)