Fig. 5

Effect of nivolumab on expression of IFN-γ and granzyme B by peripheral blood CD8⁺ cell subpopulations. Patient blood samples that were collected prior to and after completion of nivolumab treatment were immunostained with antibodies to CD8, CD4, CD3, IFN-γ, and granzyme B. The proportion of CD8^dimCD3⁺ cells (a), CD8^dimCD3⁻ (b) or CD8^hiCD3⁺ (c) that expressed IFN-γ prior to and following treatment is shown for each OCSCC patient. The proportion of CD8^dimCD3⁺ cells (d), CD8^dimCD3⁻ (e) or CD8^hiCD3⁺ (f) that expressed granzyme B prior to and following treatment is shown for each OCSCC patient Each line color indicates the same patient across all panels