Fig. 5

ROCK2 and PAK induced phosphorylation of Vimentin (Ser72 and Ser56). a Western blot analysis for p-Vimentin (Ser72, 56 and 38) expression regulated by Rac1/PAK inhibition. **P < 0.01 vs. Hypoxia (4 h). b, d Assessing ROCK1 or ROCK2 stably expressing cells by Western blot analysis. c, e Western blot analysis for p-Vimentin (Ser72, 56 and 38) expression regulated by knockdown ROCK (c) or overexpressed ROCK (e). The data are expressed as the mean ± S.E. of three independent experiments. **P < 0.01 vs. Control siRNA, or **P < 0.01 vs. Flag