Fig. 4

The effect of RhoA/ROCK and Rac1/PAK on VM formation via HIF-1α. Western blot analysis for the expression of Vimentin and E-cadherin and VE-cadherin in HIF-1α siRNA cell treated with (a) C3 transferase or Y27632, and (b) NSC23766 or IPA-3, Quantitated using Image J. Effects of (c) RhoA/ROCK and (e) Rac1/PAK on the tubular structure formation activity by 3D culture. Quantitative analysis of the mean number of tube-like structures formed from six randomly chosen areas in 3D cultures using ImageJ. Control siRNA as positive control. The data are expressed as the mean ± S.E. of three independent experiments. Original magnification, 100 ×. The scales represent 50 μm. *P < 0.05; **P < 0.01; ***P < 0.001 vs. Control siRNA. h = hours