Fig. 3

RhoA/ROCKs and Rac1/PAK act upstream of HIF-1α. a Western blot analysis for the expression of HIF-1α in MHCC97H cell lines transfected with control siRNA or HIF-1α siRNA. b Western blot analysis for RhoA/ROCK, Rac1/PAK, EMT (Vimentin and E-cadherin) and VE-cadherin (a biomarker of VM), in MHCC97H cells transfected with control siRNA or HIF-1α siRNA. The cells were exposed to hypoxia for 4 h. c Quantitated using Image J *P < 0.05; **P < 0.01 vs. control siRNA. Western blot analysis for HIF-1α expression when treated with (d) C3 transferase or Y27632, and (e) NSC23766 or IPA-3, Quantitated using Image J. The data are expressed as the mean ± S.E. of three independent experiments. **P < 0.01 vs. Hypoxia (4 h). h = hours