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Fig. 2 | BMC Cancer

Fig. 2

From: Hypoxic induction of vasculogenic mimicry in hepatocellular carcinoma: role of HIF-1 α, RhoA/ROCK and Rac1/PAK signaling

Fig. 2

Hypoxia upregulated RhoA and ROCK2 protein expression. a Effect of hypoxia on RhoA, ROCK1 and ROCK2 mRNA for 24 h. Lack of significant effect of hypoxia (4, 8 and 24 h) on RhoA/ROCK mRNA. b Time course of the effects of hypoxia on RhoA protein analysed by Western blot. c RhoA activity measured with a biochemical assay. Time course of the effects of hypoxia on ROCK1 (d) and ROCK2 (e) proteins, quantitated using Image J. ROCK2 protein was markedly increased after 4 h in hypoxia. f Effects of hypoxia on Rac1 and PAK proteins, quantitated using Image J. Normoxia condition as positive control. The data are expressed as the mean ± S.E. of three independent experiments. *P < 0.05; **P < 0.01 vs normoxia. h = hours

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BMC Cancer

ISSN: 1471-2407

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