Fig. 7
Effects of oral intake of hydrogen-rich water on xenografted mice. All nine Female BALB/c-nude mice weighing 18–25 g implanted with 1 × 107 AN3CA-LUC cells at right shoulder were fed with either hydrogen-rich water (HRW) with the concentration of 1.0 ppm or control (NC) purified water (20 mL/kg/d) for 24 days. Six for HRW-fed groups (H1, H2, H3, H4, H5, H6); Three for purified water- fed groups (P1, P2, P3); a. The tumor volume (mm3) of the HRW group was decreased compared to the control group. b. There was a trend in the relative tumor volume in the HRW group on day 1–24 compared to the control group. c. The mice weight (g) in the HRW group was diminished on day 1–10, 19–24 compared to the control group. d. Living tumor imaging after oral intake of hydrogen-rich water in xenografted mice. Photographs show tumor imaging in two groups, each containing three AN3CA-LUC cells -implanted mice per group in Day 12 (H1, H2, H3, P1, P2, P3), Day 13 (H1, H3, P2), Day 24 (H2, P1, P3). The color scale is represented by ROI = radiance (p/sec/cm2/sr). The color scale changes from blue to red, with the darker the color, the greater the tumor density. e. Mice subjected to hydrogen-rich water (HRW) displayed a decreased ROI as assessed by luminescence analysis of Total Radiant Efficiency (Day 12: HRW vs. NC, 5.90E+ 07 vs. 4.60E+ 08). Values are mean ± SD of six mice per HRW group and three mice per NC group (See Additional file 11)