Fig. 3

Fisetin treatment reduces TNBC metastasis in vivo. MDA-MB-157 and BT-549 cells were injected into two-day post-fertilization zebrafish embryos. Fish were treated either with 0.5% DMSO or with 100 μM Fisetin. Cells were allowed to migrate to the tail for 4 days. a, c Representative images of DMSO-treated fish with intravasated cells in to the tail. Green shows fish vasculature and red represents cancer cells. b, d Representative images of fisetin-treated fish. e, g Fish with less than two cells in the tail were grouped as low and fish equal to or more than two cells in the tail were grouped as high. f, h Box plots of extravasated cells in vehicle control compared to the treated groups. Note that in fisetin-treated fish with BT-549 cells, the median is at zero. A blue line in the middle panel of (a) indicates the cut-point for separating the tail from the rest of the fish. A white arrow in the same panel indicates the site of injection for tumor cells in the yolk sac. Top panels are high-resolution images for tails to highlight the migrated tumor cells in the tail region. Bottom panel shows the location of tumor cells (red) in the fish