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Fig. 6 | BMC Cancer

Fig. 6

From: Bag-1 stimulates Bad phosphorylation through activation of Akt and Raf kinases to mediate cell survival in breast cancer

Fig. 6

Subcellular localizations of Bag-1, Akt, C-Raf and Bad proteins in breast cancer cells. a. Immunocytochemical detection of Bag-1 and its pro-survival partners in MCF-7 cells. Bag-1 was stained with AlexaFlour647 goat anti-mouse (green). Phospho-AktSer473, phospho-C-RafSer338, Bad, phospho-Bad (Ser136 andSer112) as well as β-actin were stained with AlexaFlour488 goat anti-rabbit (red). Nuclei were stained with DAPI (blue). Magnification: 63X. b. Immunohistochemical detection of Bag-1 and its pro-survival partners in tumor tissues of breast cancer patients. Cytoplasmic and nuclear expression of Bag-1, Akt, phospho-Akt Ser473, phospho-C-Raf Ser338, Bad, and phospho-Bad (Ser136 andSer112) proteins were graded for patients with ER + PR + Her2-, ER + PR + Her2+ and ER-PR-Her2-subtypes. Scale bar: 100 μm

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BMC Cancer

ISSN: 1471-2407

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