Fig. 2From: Bag-1 stimulates Bad phosphorylation through activation of Akt and Raf kinases to mediate cell survival in breast cancerBag-1 expression upregulates C-Raf, B-Raf and Akt. Cells were transfected with Bag-1 vector for 48 h or Bag-1 siRNA for 24 and 48 h. Total protein was analyzed by immunoblotting with specific antibodies against C-Raf, B-Raf, Akt, phospho-C-RafSer338, phospho-B-RafS445 and phospho-AktS473Back to article page