Fig. 1
Mixed lymphocyte reaction and Th2 cell ratio. Colorimetric assays were performed to evaluate the MLR. SI (the stimulus index) = A experimental well/ Acontrol well. (A) Treated-RPMI8266 cells (treated with IFN-γ and BCGV) induced proliferation of T cells in a dose-dependent manner, and treated-RPMI8266 cells had enhanced MLR in comparison to untreated RPMI8266 and T cells; * p < 0.05. (B,C) Th2 cells after MLR for 5 d (MM: T = 1:1) stained with CD4-FITC and IL-4-PE was analyzed by FCM. Bars represent the mean ± SEM (error bars) of four separate experiments. The ratio of Th2 cell was weakly increased, but the total number of T cell was significantly increased many times by treated-myeloma cells after MLR, so the absolute number of Th2 cell produced by treated-myeloma was more significantly increased. Th2 cell number calculation formula: Absolute number Th2 = Total number T cell×Ratio Th2